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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Chromosomal rearrangements in post-chernobyl papillary thyroid carcinomas: Evaluation by spectral karyotyping and automated interphase FISH.
J. Biomed. Biotechnol. 2011:693691 (2011)
Structural genomic rearrangements are frequent findings in human cancers. Therefore, papillary thyroid (PTC) were investigated for chromosomal aberrations and rearrangements of the RET prot-oncogene. For this purpose, primary cultures from 23 PTC have been established and metaphase preparations were analysed by spectral karyotyping (SKY). In addition, interphase cell preparations of the same cases were investigated by fluorescence in situ hybridisation (FISH) for the presence of RET/PTC rearrangements using RET-specific DNA probes. SKY analysis of PTC revealed structural aberrations of chromosome 11 and several numerical aberrations with frequent loss of chromosomes 20, 21, 22. FISH analysis for RET/PTC rearrangements showed prevalence of this rearrangement in 72% (16 out of 22) of cases. However, only subpopulations of tumour cells exhibnited this rearrangement indicating genetic heterogeneity. The comparison of visual and automated scoring of FISH signals revealed concordant results in 19 out of 22 cases (87%) indicating reliable scoring results using the optimised scoring parameter for RET/PTC with the automated Metafer4 system. It can be concluded from this study that genomic rearrangements are frequent in PTC and therefore important events in thyroid carcinogenesis.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
no keywords
ISSN (print) / ISBN
1110-7243
e-ISSN
1110-7251
Zeitschrift
Journal of Biomedicine and Biotechnology
Quellenangaben
Band: 2011,
Artikelnummer: 693691
Verlag
Hindawi
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Translational Metabolic Oncology (TMO)