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Weinmeister, P.* ; Lukowski, R.* ; Linder, S.* ; Traidl-Hoffmann, C. ; Hengst, L.* ; Hofmann, F.* ; Feil, R.*

Cyclic guanosine monophosphate-dependent protein kinase I promotes adhesion of primary vascular smooth muscle cells.

Mol. Biol. Cell 19, 4434-4441 (2008)
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The cyclic guanosine monophosphate (cGMP)/cGMP-dependent protein kinase type I (cGKI) pathway regulates many cellular functions. The current study shows that 8-Br-cGMP stimulates the number of attached primary but not that of subcultured murine vascular smooth muscle cells (VSMCs). These effects of 8-Br-cGMP require the presence of cGKI. In agreement with previous studies, cGKI inhibited the number of cells in repeatedly passaged murine VSMCs. Activation of the cGMP/cGKI pathway in freshly isolated primary VSMCs slightly decreased apoptosis and strongly increased cell adhesion. The stimulation of cell adhesion by cGKI involves an inhibition of the RhoA/Rho kinase pathway and increased exposure of beta(1) and beta(3) integrins on the cell surface. Together, these results identify a novel proadhesive function of cGMP/cGKI signaling in primary VSMCs and suggest that the opposing effects of this pathway on VSMC number depend on the phenotypic context of the cells.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 1059-1524
e-ISSN 1939-4586
Zeitschrift Molecular Biology of the Cell
Quellenangaben Band: 19, Heft: 10, Seiten: 4434-4441 Artikelnummer: , Supplement: ,
Verlag American Society for Cell Biology (ASCB)
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) CCG Environmental Dermatology and Allergology (ILBD-KAU)
Institute of Epidemiology (EPI)