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Koprivnjak, J.-F.* ; Pfromm, P.H.* ; Ingall, E.* ; Vetter, T.A.* ; Schmitt-Kopplin, P. ; Hertkorn, N. ; Frommberger, M. ; Knicker, H.* ; Perdue, E.M.*

Chemical and spectroscopic characterization of marine dissolved organic matter isolated using coupled reverse osmosis-electrodialysis.

Geochim. Cosmochim. Acta 73, 4215-4231 (2009)

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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.

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The coupled reverse osmosis-electrodialysis (RO/ED) method was used to isolate dissolved organic matter (DOM) from 16 seawater samples. The average yield of organic carbon was 75 +/- 12%, which is consistently greater than the yields of organic carbon that have been commonly achieved using XAD resins, C-18 adsorbents, and cross-flow ultrafiltration. UV-visible absorbance spectra and molar C/N ratios of isolated samples were consistent with the corresponding properties of DOM in the original seawater samples, indicating that DOM samples can be isolated using the coupled RO/ED method without any bias for/against these two properties. Five of the samples were desalted sufficiently that reliable measurements of their C-13 and H-1 NMR spectra and their Fourier transform ion cyclotron resonance (FTICR) mass spectra could be obtained. The C-13 and H-1 NMR spectra of RO/ED samples differed distinctly from those of samples that have been isolated in much lower yields by other methods. In particular, RO/ED samples contained a relatively lower proportion of carbohydrate carbon and a relatively greater proportion of alkyl carbon than samples that have been isolated using cross-flow ultrafiltration. From the FTICR mass spectra of RO/ED samples, samples from the open ocean contained a much lower proportion of unsaturated compounds and a much higher proportion of fatty acids than coastal samples.

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