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Panthel, K.* ; Meinel, K.M.* ; Domenech, V.E.S.* ; Geginat, G.* ; Linkemann, K.* ; Busch, D.H. ; Rüssmann, H.*

Prophylactic anti-tumor immuntiy against a murine fibrosarcoma triggered by the Salmonella type III secretion system.

Microbes Infect. 8, 2539-2546 (2006)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The potential of an attenuated Salmonella enterica serovar Typhimurium strain as a prophylactic anti-tumor vaccine against the murine fibrosarcoma WEHI 164 was evaluated. Tumor cells were transfected with the DNA sequence encoding the MHC class I-restricted peptide p60(217-225) from Listeria monocytogenes. BALB/c mice received a single orogastric immunization with Salmonella that translocates a chimeric p60 protein via its type III secretion system. Mice were subsequently challenged subcutaneously with p60(217-225)-expressing WEHI cells. In vivo protection studies revealed that 80% of these mice remained free of the fibrosarcoma after challenge, whereas all animals of the non-vaccinated control group did develop tumor growth. In further experiments, the distribution of tetramer-positive p60(217-225)-specific effector and memory CD8 T cells after Salmonella-based immunization and tumor application was analyzed. Costaining with CD62L and CD127 revealed a predominance of p60-specific central memory and effector memory CD8 T cells in spleens, whereas in blood samples the majority of p60-specific lymphocytes belonged to effector and effector memory CD8 T cell subsets. This is the first report demonstrating that a bacterial type III secretion system can be used for heterologous antigen delivery to induce cytotoxic effector and memory CD8 T cell responses resulting in an efficient prevention of tumor growth.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Salmonella enterica serovar Typhimurium; Type III secretion system; tumor vaccine; CD8 T cell; memory T cell
Sprache englisch
Veröffentlichungsjahr 2006
HGF-Berichtsjahr 0
ISSN (print) / ISBN 1286-4579
e-ISSN 1769-714X
Zeitschrift Microbes and Infection
Quellenangaben Band: 8, Heft: 9-10, Seiten: 2539-2546 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Virology (VIRO)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) G-520100-001
DOI 10.1016/j.micinf.2006.07.004
Scopus ID 33749471430
Erfassungsdatum 2006-11-07
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