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Jasiak, A.J.* ; Hartmann, H.* ; Karakasili, E.* ; Kalocsay, M.* ; Flatley, A. ; Kremmer, E. ; Strässer, K.* ; Martin, D.E.* ; Söding, J.* ; Cramer, P.*

Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplex.

J. Biol. Chem. 283, 26423-26427 (2008)
DOI
Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Yeast RNA polymerase (Pol) II consists of a 10-subunit core enzyme and the Rpb4/7 subcomplex, which is dispensable for catalytic activity and dissociates in vitro. To investigate whether Rpb4/7 is an integral part of DNA-associated Pol II in vivo, we used chromatin immunoprecipitation coupled to high resolution tiling microarray analysis. We show that the genome-wide occupancy profiles for Rpb7 and the core subunit Rpb3 are essentially identical. Thus, the complete Pol II associates with DNA in vivo, consistent with functional roles of Rpb4/7 throughout the transcription cycle.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 0021-9258
e-ISSN 1083-351X
Zeitschrift Journal of Biological Chemistry, The
Quellenangaben Band: 283, Heft: 39, Seiten: 26423-26427 Artikelnummer: , Supplement: ,
Verlag American Society for Biochemistry and Molecular Biology
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Immunology (IMI)