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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Photoactivation of the CreER T² recombinase for conditional site-specific recombination with high spatiotemporal resolution.
Zebrafish 7, 199-204 (2010)
We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one- or two-photon illumination was used to restore Cre activity of the CreER(T2) fusion protein in specific target cells. This method labels single cells irreversibly by inducing recombination in an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution in the control of gene expression. This technique could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration, or carcinogenesis) with high spatiotemporal resolution (single cell and 10-min scales).
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Transgenic zebrafish; Mice; Expression; System; Tool
ISSN (print) / ISBN
1545-8547
e-ISSN
1557-8542
Zeitschrift
Zebrafish
Quellenangaben
Band: 7,
Heft: 2,
Seiten: 199-204
Verlag
Mary Ann Liebert
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed