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Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Haemopoietic Inductive Capacity of Irradiated Stromal Cell Layers in Human Micro Long-Term Bone Marrow Cultures.
Cell Prolif. 21, 411-417 (1989)
In a micro long-term bone marrow culture (LTBMC) system the effects of irradiation on confluent stromal cell layers were studied. In order to individually analyse the number of granulocyte-macrophage colony-forming cells (GM-CFC) per LTBMC a miniaturized human GM-CFC assay was established. The normalized GM-CFC numbers in the micro-assay compared well with data by the conventional GM-CFC assay. Pre-formed stromal cell layers were irradiated with doses up to 20 Gy and subsequently recharged with allogeneic bone marrow cells (BMC). Immediately before recharge the BMC were stromal cell-depleted by nylon wool filtration. When stromal cell-depleted BMC were inoculated on empty culture dishes, in vitro haemopoiesis rapidly declined. Sustained GM-CFC production, however, was seen when these cells were used as a second inoculum. It is concluded that irradiation doses of up to 20 Gy do not cause alteration of the haemopoietic inductive capacity of confluent stromal cell layers.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
ISSN (print) / ISBN
0008-8730
e-ISSN
1365-2184
Zeitschrift
Cell Proliferation
Quellenangaben
Band: 21,
Seiten: 411-417
Verlag
Blackwell
Verlagsort
Oxford [u.a.] ; Berlin ; Vienna
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed