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Construction and characterization of a packaging cell line for MMTV-based conditional retroviral vectors.
Biochem. Biophys. Res. Commun. 159, 1191-1198 (1989)
A chimeric provirus in which the 5'LTR of a complete biologically active Mouse Mammary Tumour Virus (MMTV) proviral DNA has been replaced with the Rous Sarcoma Virus LTR has been constructed. Upon transfection into permissive cells, this provirus directs the synthesis of the MMTV gag and env structural proteins, but is impaired in packaging of the RNAs that encode these proteins. Supertransfection of these cells with MMTV based vector constructs results in the production of infectious recombinant virus at a higher efficiency than with previously described helper cell lines. Such a retroviral vector system based on MMTV will allow the study of the effects of conditional expression of inserted genes upon infected cells.
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Anmerkungen
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Sprache
englisch
Veröffentlichungsjahr
1989
HGF-Berichtsjahr
0
ISSN (print) / ISBN
0006-291X
e-ISSN
1090-2104
Quellenangaben
Band: 159,
Heft: 3,
Seiten: 1191-1198
Verlag
Elsevier
Begutachtungsstatus
Peer reviewed
Institut(e)
Departments & Institutes
PubMed ID
2539144
Erfassungsdatum
1989-12-31