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Salmons, B. ; Moritz-Legrand, S. ; Garcha, I. ; Günzburg, W.H.

Construction and characterization of a packaging cell line for MMTV-based conditional retroviral vectors.

Biochem. Biophys. Res. Commun. 159, 1191-1198 (1989)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A chimeric provirus in which the 5'LTR of a complete biologically active Mouse Mammary Tumour Virus (MMTV) proviral DNA has been replaced with the Rous Sarcoma Virus LTR has been constructed. Upon transfection into permissive cells, this provirus directs the synthesis of the MMTV gag and env structural proteins, but is impaired in packaging of the RNAs that encode these proteins. Supertransfection of these cells with MMTV based vector constructs results in the production of infectious recombinant virus at a higher efficiency than with previously described helper cell lines. Such a retroviral vector system based on MMTV will allow the study of the effects of conditional expression of inserted genes upon infected cells.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 0006-291X
e-ISSN 1090-2104
Zeitschrift Biochemical and Biophysical Research Communications
Quellenangaben Band: 159, Heft: 3, Seiten: 1191-1198 Artikelnummer: , Supplement: ,
Verlag Elsevier
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Departments & Institutes