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Klose, U. ; Thierau, D. ; Veser, U. ; Schwarz, L.R.

Stimulation of DNA-Synthesis in Carcinogen-Induced Diploid Hepatocytes in vitro.

Carcinogenesis 11, 1479-1483 (1990)
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Diploid hepatocytes induced by a combination of diethylnitrosamine and 2-acetylaminofluorene were isolated and separated from polyploid hepatocytes by centrifugal elutriation. The diploid and polyploid cell fractions were approximately 90% pure and contained between 1 and 1.5 x 10(7) cells. When kept in monolayer cultures both cell populations responded to the mitogenic effect of EGF and insulin. However, the percentage of labelled nuclei was higher in predominantly diploid compared to predominantly tetraploid hepatocyte cultures at all epidermal growth factor (EGF) concentrations used in this study. At 10 ng EGF/ml and 10 mU insulin/ml the labelling index was twice as high in the diploid liver cells. Further work is required to show the relevance of the stronger response of the diploid cell fraction to mitogens in the process of carcinogenesis.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Sprache
Veröffentlichungsjahr 1990
HGF-Berichtsjahr 1990
ISSN (print) / ISBN 0143-3334
e-ISSN 1460-2180
Zeitschrift Carcinogenesis
Quellenangaben Band: 11, Heft: 9, Seiten: 1479-1483 Artikelnummer: , Supplement: ,
Verlag Oxford University Press
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Toxicology and Pharmacology (TOX)
DOI 10.1093/carcin/11.9.1479
PubMed ID 2205406
Erfassungsdatum 1990-12-31
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