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Two-dimensional Electrophoresis of Dog Bronchoalveolar Lavage Fluid Proteins.

Electrophoresis 11, 510-513 (1990)
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Proteins of dog bronchoalveolar lavage fluid, obtained by washing the epithelial lining layer of lungs with phosphate-buffered saline, were separated by two-dimensional electrophoresis. Due to the low protein and high salt content of the bronchoalveolar lavage fluid, samples had to be concentrated and desalted. Following electrophoresis the protein spots were visualized by silver staining. Comparing the two-dimensional protein patterns of bronchoalveolar lavage fluid with that from serum, several lungspecific proteins were detected. The most prominent protein, most probably a surfactant-associated protein, showed isoforms with isoelectric points in the range of pH 4.2–4.8, and a molecular mass of 32 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis after reduction with dithiothreitol.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Sprache englisch
Veröffentlichungsjahr 1990
HGF-Berichtsjahr 0
ISSN (print) / ISBN 0173-0835
e-ISSN 1522-2683
Zeitschrift Electrophoresis
Quellenangaben Band: 11, Heft: 6, Seiten: 510-513 Artikelnummer: , Supplement: ,
Verlag Wiley
Begutachtungsstatus Peer reviewed
Erfassungsdatum 1990-12-31