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Aubele, M. ; Vidojkovic, S. ; Braselmann, H. ; Ritterswürden, D. ; Auer, G.* ; Atkinson, M.J. ; Tapio, S. ; Höfler, H. ; Rauser, S. ; Bartlett, J.M.*

Overexpression of PTK6 (breast tumor kinase) protein - a prognostic factor for long-term breast cancer survival - is not due to gene amplification.

Virchows Arch. 455, 117-123 (2009)
Verlagsversion DOI PMC
Free by publisher
In a previous retrospective study, we demonstrated the prognostic value of protein tyrosine kinase 6 (PTK6) protein expression in breast carcinomas. Here, we analyzed PTK6 gene amplification using fluorescence in situ hybridization technique in a cohort of 426 invasive breast carcinomas and compared it with PTK6 expression level as well as with the clinical outcome of patients. Forty-five percent of tumors show increased PTK6 gene copy numbers when compared to normal tissue. Most of these, however, were related to chromosome 20 polysomy (30%), while gene amplification accounted for only 15%. Only "low level" amplification of the PTK6 gene, with up to eight signals per nucleus, was found. The PTK6 cytogenetic status (normal, gene amplification, polysomy 20) was not associated with histopathological parameters or with the protein expression of HER receptors. No statistical association was identified between PTK6 gene status and expression level. Further, the PTK6 gene status does not influence the disease-free survival of patients at a parts per thousand yen240 months. Based on these results, we state that the PTK6 overexpression is not essentially attributed to gene amplification, and the PTK6 protein expression-but not gene status-is of prognostic value in breast carcinomas. PTK6 protein overexpression may result from polysomy 20 in a minority of the tumors. In a marked proportion of tumors, however, the overexpression is likely to be caused by posttranscriptional regulation mechanisms.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter PTK6 (BRK); Gene amplification; FISH; Overexpression; Breast cancer; Prognosis; brk tyrosine kinase; in-situ hybridization; poor-prognosis; expression; carcinomas; cells; brk/sik; association; esophageal; oncogenes
ISSN (print) / ISBN 0042-6423
e-ISSN 1432-2307
Zeitschrift Virchows Archiv
Quellenangaben Band: 455, Heft: 2, Seiten: 117-123 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Heidelberg
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pathology (PATH)
Institute of Molecular Radiation Biology (IMS)
Institute of Radiation Biology (ISB)
Research Unit Analytical Pathology (AAP)