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Schmid, J. ; Aubele, M. ; Jütting, U. ; Rodenacker, K. ; Luz, A. ; Erfle, V. ; Burger, G.

Computer-assisted Imaging Cytometry of Nuclear Chromatin Reveals Bone Tumor Virus Infection and Neoplastic Transformation of Adherent Osteoblast-like Cells.

Biochem. Biophys. Res. Commun. 164, 728-735 (1989)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Established osteoblast-like (OB) cells infected with the bone tumor-inducing C-type retrovirus OA MuLV remained nontumorigenic over 104 cell culture passages. DNA histograms revealed a new cell population with a stem line peak at 5c. A second OA MuLV-infected OB cell line underwent neoplastic transformation with increasing passage level. These cells showed diffuse aneuploidy. Stepwise linear discriminant analysis of the chromatin structure of control, OA MuLV-infected, and FBR osteosarcoma virus-transformed cell lines resulted in various levels of discrimination ranging between 79.6% for control cells versus nontumorigenic OA MuLV-infected cells, and 96.6% for nontumorigenic OA MuLV-infected cells versus FBR osteosarcoma virus-transformed cells. OA MuLV-infected tumorigenic cells and FBR osteosarcoma virus-transformed cells were discriminated at a 93.6% level.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 0006-291X
e-ISSN 1090-2104
Zeitschrift Biochemical and Biophysical Research Communications
Quellenangaben Band: 164, Heft: 2, Seiten: 728-735 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Radiation Protection (ISS)