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möglich sobald bei der ZB eingereicht worden ist.
Centromere Detection in Vinblastine- and Radiation-induced Micronuclei of Cytokinesis-blocked Mouse Cells Using in Situ Hybridization with a Mouse gamma (major) Satellite DNA Probe.
Environ. Mol. Mutagen. 19, 1-6 (1992)
Non-isotopic in situ hybridization using a mouse gamma (major) satellite probe DNA was applied to detect centromeres in micronuclei, which were induced in vitro in mouse liver cells by ionizing radiation and by vinblastine sulfate. In a cytokinesis-blocked micronucleus assay a dose-dependent induction of micronuclei was found for both agents. After vinblastine exposure the observed micronuclei showed centromere-positive hybridization signals in an order of magnitude of 70-90%, but after radiation exposure the magnitude was only 10-20%. Since the in situ hybridization technique detects centromeric DNA directly, it can be used in a cytokinesis-blocked micronucleus assay for a rapid and reliable discrimination between aneuploidy-inducing and clastogenic agents.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
ISSN (print) / ISBN
0192-2521
e-ISSN
1098-2280
Zeitschrift
Environmental and Molecular Mutagenesis
Quellenangaben
Band: 19,
Heft: 1,
Seiten: 1-6
Verlag
Wiley
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Radiation Biology (ISB)