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Salassidis, K. ; Huber, R. ; Zitzelsberger, H. ; Bauchinger, M.

Centromere Detection in Vinblastine- and Radiation-induced Micronuclei of Cytokinesis-blocked Mouse Cells Using in Situ Hybridization with a Mouse gamma (major) Satellite DNA Probe.

Environ. Mol. Mutagen. 19, 1-6 (1992)
Verlagsversion DOI
Non-isotopic in situ hybridization using a mouse gamma (major) satellite probe DNA was applied to detect centromeres in micronuclei, which were induced in vitro in mouse liver cells by ionizing radiation and by vinblastine sulfate. In a cytokinesis-blocked micronucleus assay a dose-dependent induction of micronuclei was found for both agents. After vinblastine exposure the observed micronuclei showed centromere-positive hybridization signals in an order of magnitude of 70-90%, but after radiation exposure the magnitude was only 10-20%. Since the in situ hybridization technique detects centromeric DNA directly, it can be used in a cytokinesis-blocked micronucleus assay for a rapid and reliable discrimination between aneuploidy-inducing and clastogenic agents.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Sprache englisch
Veröffentlichungsjahr 1992
HGF-Berichtsjahr 0
ISSN (print) / ISBN 0192-2521
e-ISSN 1098-2280
Zeitschrift Environmental and Molecular Mutagenesis
Quellenangaben Band: 19, Heft: 1, Seiten: 1-6 Artikelnummer: , Supplement: ,
Verlag Wiley
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Radiation Biology (ISB)
Erfassungsdatum 1992-12-31
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