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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Accumulation of p53 Protein as an Indicator for p53 Gene Mutation in Breast Cancer: Occurence of False-Positives and False-Negatives.
Diagn. Mol. Pathol. 2, 36-41 (1993)
Accumulation of p53 protein resulting in levels detectable by immunohistochemistry (IHC) has been proposed as an indicator of mutation of the p53 gene. We have investigated a panel of 23 fresh-frozen breast cancers by IHC (PAb 1801), Southern and Northern blot analysis, and direct sequencing of the mutation hot spot regions (exons 5-8) of the p53 gene. Three tumors (13%) showed an intense nuclear staining in the majority of malignant cells, but only one of these showed a mutation of the p53 gene (codon 237, Arg to His). Furthermore, a mutation (5-bp deletion) was identified in a tumor that showed no p53 immunoreactivity. Our results indicate that accumulation of p53 protein, as detectable by IHC, is not a reliable indicator for p53 gene mutation in human breast cancer.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
ISSN (print) / ISBN
1052-9551
e-ISSN
1533-4066
Zeitschrift
Diagnostic Molecular Pathology
Quellenangaben
Band: 2,
Heft: 1,
Seiten: 36-41
Verlag
Lippincott Williams & Wilkins
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Pathology (PATH)