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Molecular Cloning, Sequence Analysis and Elicitor-/ozone-induced Accumulation of Cinnamyl Alcohol Dehydrogenase from Norway Spruce (Picea abies L.).
Plant Mol. Biol. 23, 145-156 (1993)
Cinnamyl alcohol dehydrogenase (CAD) is an enzyme involved in lignin biosynthesis. We have previously isolated pure CAD enzyme from Norway spruce (Picea abies L.) cell culture. Here we report on partial protein sequences of the 42 kDa CAD polypeptide. A cDNA encoding CAD was isolated from the spruce cell culture. The open reading frame of a full-length cDNA coded for a 357 amino acid polypeptide with a calculated M(r) of 38,777 Da. The identity of the deduced polypeptide was verified by comparison with amino acid sequences of tryptic peptides from the purified enzyme. Southern blot analysis showed the presence of only one gene for CAD. Sequence comparison with CAD from tobacco and with a N-terminal protein sequence from loblolly pine CAD showed an identity of 69.7% and 91.5%, respectively. Treatment of spruce cell cultures with elicitor, as well as of seedlings with ozone both markedly increased the CAD mRNA level.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
CAD; DNA; sequence; elicitor; lignin; ozone; spruce
ISSN (print) / ISBN
0167-4412
e-ISSN
0167-4412
Zeitschrift
Plant Molecular Biology
Quellenangaben
Band: 23,
Seiten: 145-156
Verlag
Springer
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Biochemical Plant Pathology (BIOP)