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Michalke, B. ; Schramel, P.

Iodine speciation in biological samples by capillary electrophoresis : inductively coupled plasma mass spectrometry.

Electrophoresis 20, 2547-2553 (1999)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A hyphenation of capillary electrophoresis (CE) to inductively coupled plasma mass spectrometry (ICP-MS) was employed for the speciation of iodine. The separation method used a buffer sandwich of phosphate (pH 2.3), NaOH, sodium dodecyl sulfate (SDS) and borate buffer (pH 8.3) for stacking, aiming at sufficient separation of iodide, iodate, thyroxine (T4) and triiodothyronine (T3). These four iodine species were separated within 15 min and subsequently detected during a pressure-driven detection step (baseline-separated) at 19.5, 29.1, 36.6 and 42.2 s. The detection limits were determined at 0.08 μg I/L (iodide), 0.3 μg I/L (iodate), 3.5 μg I/L (thyroxine) and 2.5 μg I/L (triiodothyronine). This method was applied on iodine speciation in human serum (“healthy” and after thyroid gland operation) and urine. The serum from the healthy person contained iodide (13 μg I/L), T4 (61 μg I/L) and T3 (7.5 μg I/L), whereas the serum from the thyroid-operated person lacked T3. As no “free” I-hormones are known in serum, the role of the thyroid hormone binding globulin (TBG) was investigated. We found that spiked T4 or T3 immediately bound to TBG. Investigations on human urine showed only a peak for iodide.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Iodine speciation; Human serum; Capillary electrophoresis; Inductively coupled plasma-mass spectrometry hyphenation
ISSN (print) / ISBN 0173-0835
e-ISSN 1522-2683
Zeitschrift Electrophoresis
Quellenangaben Band: 20, Heft: , Seiten: 2547-2553 Artikelnummer: , Supplement: ,
Verlag Wiley
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Ecological Chemistry (IOEC)