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Hagn, A. ; Wallisch, S. ; Radl, V. ; Munch, J.-C. ; Schloter, M.

A new cultivation independent approach to detect and monitor common Trichoderma species in soils.

J. Microbiol. Methods 69, 86-92 (2007)
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A set of primers was developed for the detection, identification and quantification of common Trichoderma species in soil samples. Based on a broad range master alignment primers were derived to amplify an approximate 540 bp fragment comprising the internal transcribed spacer region 1 (ITS 1), 5.8S rDNA and internal transcribed spacer region 2 (ITS 2) from all taxonomic Clades of the genus Trichoderma.The primer set was applied to test strains as well as community DNA isolated from arable and forest soil. For all tested isolates the corresponding internal transcribed spacer regions of Trichoderma spp. strains were amplified, but none of non-Trichoderma origin. PCR with community DNA from soil yielded products of the expected size. Analysis of a clone library established for an arable site showed that all amplified sequences originated exclusively from Trichoderma species mainly being representatives of the Clades Hamatum, Harzianum and Pachybasioides and comprising most of the species known for biocontrol ability.In a realtime PCR approach the primer set uTf/uTr also proved to be a suitable system to quantify DNA of Trichoderma spp. in soils.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Environmental samples; ITS region; qPCR; Trichoderma
ISSN (print) / ISBN 0167-7012
e-ISSN 1872-8359
Zeitschrift Journal of Microbiological Methods
Quellenangaben Band: 69, Heft: , Seiten: 86-92 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Amsterdam
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Soil Ecology (IBOE)