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Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov.
Int. J. Syst. Evol. Microbiol. 50, 2207-2223 (2000)
Verlagsversion Volltext
PMC
A large collection of bacterial strains, immunotrapped from soil and from the wheat rhizoplane, was subjected to polyphasic taxonomy by examining various pheno- and genotypic parameters. Strains were grouped on (inter) repetitive extragenic palindromic DNA (REP) PCR profiles at the intraspecies level. Pheno- and genotypic characters were assessed for representatives from 13 different REP groups. Strains of nine REP groups constituting two physiological BIOLOG clusters fell in the coherent DNA-DNA reassociation group of Ochrobactrum anthropi. Strains of two REP groups constituting a separate BIOLOG cluster fell in the coherent DNA-DNA reassociation group of Ochrobactrum intermedium. Additional phenotypic characters differentiating O. anthropi and O. intermedium were found. REP group K strains constituted a different BIOLOG cluster, a separate DNA-DNA reassociation group and a distinct phylogenetic lineage in 165 rDNA homology analysis, indicating that REP group K strains represent a new species. Diagnostic phenotypic characters were found. Closest relatives were Ochrobactrum species. The name Ochrobactrum grignonense sp. nov. is proposed (type strain OgA9aT = LMG 18954T = DSM 13338T). REP group J strains again constituted a different BIOLOG cluster, a separate DNA-DNA reassociation group and showed, as a biological particularity, a strict preference for the rhizoplane as habitat. Diagnostic phenotypic characters were found. This indicated that REP group J strains represent a further new species, although phylogenetic analyses using 16S rDNA homology were not able to separate the cluster of REP group J sequences significantly from 16S rDNA sequences of Ochrobactrum anthropi. The name Ochrobactrum tritici sp. nov. is proposed (type strain SCII24T = LMG 18957T = DSM 13340T).
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Ochrobactrum; immunotrapping from bulk soil and wheat rhizoplane; polyphasic taxonomy; geno- and phenotyping; repetitive extragenic palindromic DNA; 16S RIBOSOMAL-RNA; DEOXYRIBONUCLEIC-ACID; PHYLOGENETIC ANALYSIS; SPECIES DEFINITION; GROUP-VD; BACTERIAL; DNA; AEROMONAS; SEQUENCES; BRUCELLA
ISSN (print) / ISBN
1466-5026
e-ISSN
1466-5034
Quellenangaben
Band: 50,
Seiten: 2207-2223
Verlag
SGM
Verlagsort
Reading
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed