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Schloter, M. ; Bode, W. ; Hartmann, A.

Characterization and application of a strain-specific monoclonal antibody against the rhizosphere bacterium Azospirillum brasilense Wa5.

Monoclon. Antib. Immunodiagn. Immunother. 16, 183-187 (1997)
DOI PMC
A hybridoma cell line producing a rat monoclonal antibody (MAb Bo-33) directed against lipopolysaccharide of Azospirillum brasilense Wa5 has been established and characterized. Whole bacteria were used as immunogens. The number of antigens per cell was about 1500. The number of antigens per cell of reisolates from the rhizosphere of what was similar to the number of antigens of bacteria cultivated in rich medium. The sensitivity of detection using MAb Bo-33 was about 100 bacteria/ml. Therefore, the MAb was suitable for in situ immunofluorescence detection and a sensitive direct quantification of Azospirillum brasilense Wa5 in rhizosphere extracts.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 0272-457X
e-ISSN 1557-8097
Zeitschrift Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Quellenangaben Band: 16, Heft: 2, Seiten: 183-187 Artikelnummer: , Supplement: ,
Verlag Mary Ann Liebert
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Soil Ecology (IBOE)
Research Unit Microbe-Plant Interactions (AMP)