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T cell activation induces proteasomal degradation of Argonaute and rapid remodeling of the microRNA repertoire.
J. Exp. Med. 210, 417-432 (2013)
Verlagsversion Volltext
DOI
PMC
Activation induces extensive changes in the gene expression program of naive CD4(+) T cells, promoting their differentiation into helper T cells that coordinate immune responses. MicroRNAs (miRNAs) play a critical role in this process, and miRNA expression also changes dramatically during T cell differentiation. Quantitative analyses revealed that T cell activation induces global posttranscriptional miRNA down-regulation in vitro and in vivo. Argonaute (Ago) proteins, the core effector proteins of the miRNA-induced silencing complex (miRISC), were also posttranscriptionally down-regulated during T cell activation. Ago2 was inducibly ubiquitinated in activated T cells and its down-regulation was inhibited by the proteasome inhibitor MG132. Therefore, activation-induced miRNA down-regulation likely occurs at the level of miRISC turnover. Measurements of miRNA-processing intermediates uncovered an additional layer of activation-induced, miRNA-specific transcriptional regulation. Thus, transcriptional and posttranscriptional mechanisms cooperate to rapidly reprogram the miRNA repertoire in differentiating T cells. Altering Ago2 expression in T cells revealed that Ago proteins are limiting factors that determine miRNA abundance. Naive T cells with reduced Ago2 and miRNA expression differentiated more readily into cytokine-producing helper T cells, suggesting that activation-induced miRNA down-regulation promotes acquisition of helper T cell effector functions by relaxing the repression of genes that direct T cell differentiation.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Posttranscriptional Regulation ; Transcription Factors ; Immune-responses ; Ubiquitin Ligase ; Gene-expression ; Rna-binding ; Differentiation ; Biogenesis ; Proteins ; Pathway
ISSN (print) / ISBN
0022-1007
e-ISSN
1540-9538
Zeitschrift
Journal of Experimental Medicine
Quellenangaben
Band: 210,
Heft: 2,
Seiten: 417-432
Verlag
Rockefeller University Press
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed