Startseite
English
Erweiterte Suche
Durchblättern nach ...
Publikationen im Überblick
Ansprechpartner
Hilfe
DOI
 |
|
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Construction of umu-fhuA operon fusions to detect genotoxic potential by an antibody-cell surface reaction.
Environ. Toxicol. 9, 285-291 (1994)
For the environmental monitoring of genotoxic effects of chemicals, a new test system was developed using immunochemical detection of a cell surface reporter protein of Escherichia coli. A umu-fhuA operon fusion was constructed on plasmid pST12, which is inducible via the SOS system by the mutagenic agent methyl-methane-sulfonate (MMS). The expression of the outer membrane protein FhuA as a reporter was detectable on the cell surface with a monoclonal anti-FhuA antibody. Enzymelinked immunosorbent assay tests and immunofluorescence microscopy revealed that the operon fusion of PST12 led to specific and strong induction of the reporter protein FhuA after genotoxic treatment with MMS.
Altmetric
Weitere Metriken?
Zusatzinfos bearbeiten
[➜Einloggen]
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
ISSN (print) / ISBN
0884-8181
e-ISSN
1098-2256
Zeitschrift
Environmental Toxicology
Quellenangaben
Band: 9,
Heft: 4,
Seiten: 285-291
Verlag
Wiley
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed