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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Multicolor chromosome painting in diagnostic and research applications.
Chromosome Res. 12, 15-23 (2004)
For many years whole chromosome painting probes have been the work-horses in a large variety of clinical and research molecular cytogenetic applications. In recent years painting probes have been complemented by an increasing number of further region-specific probes, which allow the specific staining of centromeres, subtelomeres or other regions within the genome. This development of new probe sets was greatly facilitated by the Human Genome Project from which well-characterized probes for any region within the genome have emerged. Furthermore, the evolution of different multicolor fluorescence in situ hybridization (FISH) technologies now allows the cohybridization of multiple DNA-probes of different colors. These developments have paved the way for FISH-based automated karyotyping or the simultaneous analysis of multiple defined regions within the genome. Using appropriate instrumentation and image processing, the analysis can be performed two-dimensionally on metaphase spreads or three-dimensionally in intact interphase nuclei. Here we summarize some of the most recent developments and discuss the application of painting probes in different scenarios.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
centromere; chromosome painting; clinical cytogenetics; DNA probes; in situ hybridization; telomere
ISSN (print) / ISBN
0967-3849
e-ISSN
1573-6849
Zeitschrift
Chromosome Research
Quellenangaben
Band: 12,
Heft: 1,
Seiten: 15-23
Verlag
Springer
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Human Genetics (IHG)