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Clonal chromosomal aberrations in simian virus 40-transfected human thyroid cells and in derived tumors developed after in vitro irradiation.
Int. J. Cancer 96, 166-177 (2001)
In vitro model cell systems are important tools for studying mechanisms of radiation-induced neoplastic transformation of human epithelial cells. In our study, the human thyroid epithelial cell line HTori-3 was analyzed cytogenetically following exposure to different doses of alpha- and gamma-irradiation and subsequent tumor formation in athymic nude mice. Combining results from G-banding, comparative genomic hybridization, and spectral karyotyping, chromosome abnormalities could be depicted in the parental line HTori-3 and in nine different HTori lines established from the developed tumors. A number of chromosomal aberrations were found to be characteristic for simian virus 40 immortalization and/or radiation-induced transformation of human thyroid epithelial cells. Common chromosomal changes in cell lines originating from different irradiation experiments were loss of 8q23 and 13cen-q21 as well as gain of 1q32-qter and 2q11.2-q14.1. By comparison of chromosomal aberrations in cell lines exhibiting a different tumorigenic behavior, cytogenetic markers important for the tumorigenic process were studied. It appeared that deletions on chromosomes 9q32-q34 and 7q21-q31 as well as an increased copy number of chromosome 20 were important for the tumorigenic phenotype. A comparative breakpoint analysis of the marker chromosomes found and those observed in radiation-induced childhood thyroid tumors from Belarus revealed a coincidence for a number of chromosome bands. Thus, the data support the usefulness of the established cell system as an in vitro model to study important steps during radiation-induced malignant transformation in human thyroid cells.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
in vitro model cell system; radiation-induced tumorigenesis; chromosomal aberration; comparative genomic hybridization; spectral karyotyping; COMPARATIVE GENOMIC HYBRIDIZATION; RADIATION-INDUCED TRANSFORMATION; COPY NUMBER CHANGES; EPITHELIAL-CELLS; CELLULAR SENESCENCE; NEOPLASTIC TRANSFORMATION; INDEFINITE DIVISION; IONIZING-RADIATION; ALPHA-PARTICLES; SOLID TUMORS
ISSN (print) / ISBN
0020-7136
e-ISSN
1097-0215
Zeitschrift
International Journal of Cancer
Quellenangaben
Band: 96,
Heft: 3,
Seiten: 166-177
Verlag
Wiley
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Molecular Radiation Biology (IMS)