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Continuous long-term detection of live cell surface markers by ‘in culture’ antibody staining.
Protoc. Exch., DOI: 10.1038/protex.2011.205 (2011)
Long-term observation of cell behavior at the single cell level is a crucial prerequisite for understanding molecular cell fate control. This is of particular importance when analyzing highly dynamic and heterogeneous developmental and stem cell systems. The required observations are possible by time-lapse microscopy, but reliable identification of specific cell types and maturation stages usually requires detection of molecular marker expression. However, only few of the necessary cell or animal lines expressing genetically encoded fluorescent markers exist, and the generation of novel reporter lines is laborious and slow. Here, we describe long-term antibody staining in living cell cultures as an easy, robust, flexible and fast way of continuous real-time marker detection on living cells. This approach greatly simplifies the live identification of specific cell types in heterogeneous and dynamic culture systems.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
antibody; live cell; imaging; microscopy
Zeitschrift
Protocol Exchange
Quellenangaben
Seiten: DOI: 10.1038/protex.2011.205
Verlag
Nature Publishing Group
Verlagsort
New York, NY
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Research Unit Stem Cell Dynamics (SCD)