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Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.
EMBO Rep. 8, 1052-1060 (2007)
Members of the Argonaute (Ago) protein family associate with small RNAs and have important roles in RNA silencing. Here, we analysed Ago1- and Ago2-containing protein complexes in human cells. Separation of Ago-associated messenger ribonucleoproteins (mRNPs) showed that Ago1 and Ago2 reside in three complexes with distinct Dicer and RNA-induced silencing complex activities. A comprehensive proteomic analysis of Ago-containing mRNPs identified a large number of proteins involved in RNA metabolism. By using co-immunoprecipitation experiments followed by RNase treatment, we biochemically mapped interactions within Ago mRNPs. Using reporter assays and knockdown experiments, we showed that the putative RNA-binding protein RBM4 is required for microRNA-guided gene regulation.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Argonaute proteins; gene silencing; microRNA; RNA interference
ISSN (print) / ISBN
1469-221X
e-ISSN
1469-3178
Zeitschrift
EMBO Reports
Quellenangaben
Band: 8,
Heft: 11,
Seiten: 1052-1060
Verlag
EMBO Press
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Molecular Immunology (IMI)