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Forst, A.H.* ; Karlberg, T.* ; Herzog, N.* ; Thorsell, A.-G.* ; Gross, A.* ; Feijs, K.L.H.* ; Verheugd, P.* ; Kursula, P.* ; Nijmeijer, B.* ; Kremmer, E. ; Kleine, H.* ; Ladurner, A.G.* ; Schüler, H.* ; Lüscher, B.*

Recognition of mono-ADP-ribosylated ARTD10 substrates by ARTD8 macrodomains.

Structure 21, 462-475 (2013)
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ADP-ribosyltransferases (ARTs) catalyze the transfer of ADP-ribose from NAD(+) onto substrates. Some ARTs generate in an iterative process ADP-ribose polymers that serve as adaptors for distinct protein domains. Other ARTs, exemplified by ARTD10, function as mono-ADP-ribosyltransferases, but it has been unclear whether this modification occurs in cells and how it is read. We observed that ARTD10 colocalized with ARTD8 and defined its macrodomains 2 and 3 as readers of mono-ADP-ribosylation both in vitro and in cells. The crystal structures of these two ARTD8 macrodomains and isothermal titration calorimetry confirmed their interaction with ADP-ribose. These macrodomains recognized mono-ADP-ribosylated ARTD10, but not poly-ADP-ribosylated ARTD1. This distinguished them from the macrodomain of macroH2A1.1, which interacted with poly- but not mono-ADP-ribosylated substrates. Moreover, Ran, an ARTD10 substrate, was also read by ARTD8 macrodomains. This identifies readers of mono-ADP-ribosylated proteins, defines their structures, and demonstrates the presence of this modification in cells.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Polymerase-activity ; Dna-damage ; Poly(adp-ribose) ; Proteins ; Domains ; Binding ; Mechanism ; Chromatin ; Parp1 ; Ubiquitination
Sprache englisch
Veröffentlichungsjahr 2013
HGF-Berichtsjahr 2013
ISSN (print) / ISBN 0969-2126
e-ISSN 1878-4186
Zeitschrift Structure
Quellenangaben Band: 21, Heft: 3, Seiten: 462-475 Artikelnummer: , Supplement: ,
Verlag Cell Press
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Immunology (IMI)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) G-501793-001
PubMed ID 23473667
DOI 10.1016/j.str.2012.12.019
WOS ID WOS:000315975100017
Scopus ID 84874456032
Erfassungsdatum 2013-04-04
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