PuSH - Publikationsserver des Helmholtz Zentrums München: Continuous single-cell imaging of blood generation from haemogenic endothelium.

Informationen

Hinweise zu Qualitätskriterien von Zeitschriften

Open-Access-Richtlinie der Helmholtz-Gemeinschaft 2016

CC Licencences

Metriken für Publikationen

Navigation

Startseite

English

EVA: Veröffentlichen

Neuer EVA Antrag

Recherche

Erweiterte Suche

Durchblättern nach ...

... HMGU-Autoren/Konsortien

... Organisationsstruktur

... Zeitschriften

... Publikationstypen

... Forschungsdaten

... Arbeitsgruppen

... Erscheinungsjahr

Publikationen im Überblick

Statistik

HGF Fortschrittsbericht

OA Publikationen

Eintragen

Neue Publikation eintragen

Neue Publikation holen aus...

...EVA

Fehlende Publikation melden

Highlights

Suche

Hilfe & Kontakt

Ansprechpartner

Hilfe

Datenschutz

Helmholtz Open Science

Bibliometrische Indikatoren

SHERPA/RoMEO

DOAJ

Export:

Text

Endnote (RIS) BIB

BibTeX

Eilken, H.M. ; Nishikawa, S.-I.* ; Schroeder, T.

Continuous single-cell imaging of blood generation from haemogenic endothelium.

Nature 457, 896-900 (2009)

DOI

PMC

Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.

Abstract
Metriken
Zusatzinfos

Despite decades of research, the identity of the cells generating the first haematopoietic cells in mammalian embryos is unknown(1). Indeed, whether blood cells arise from mesodermal cells, mesenchymal progenitors, bipotent endothelial - haematopoietic precursors or haemogenic endothelial cells remains controversial(2-9). Proximity of endothelial and blood cells at sites of embryonic haematopoiesis, as well as their similar gene expression, led to the hypothesis of the endothelium generating blood. However, owing to lacking technology(10) it has been impossible to observe blood cell emergence continuously at the single- cell level, and the postulated existence of haemogenic endothelial cells remains disputed(1). Here, using new imaging and cell- tracking methods, we show that embryonic endothelial cells can be haemogenic. By continuous long- term single- cell observation of mouse mesodermal cells generating endothelial cell and blood colonies, it was possible to detect haemogenic endothelial cells giving rise to blood cells. Living endothelial and haematopoietic cells were identified by simultaneous detection of morphology and multiple molecular and functional markers. Detachment of nascent blood cells from endothelium is not directly linked to asymmetric cell division, and haemogenic endothelial cells are specified from cells already expressing endothelial markers. These results improve our understanding of the developmental origin of mammalian blood and the potential generation of haematopoietic stem cells from embryonic stem cells.

Impact Factor

Scopus SNIP

Web of Science
Times Cited

Scopus
Cited By

Altmetric

31.434

9.030

333

429