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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Dynamic force microscopy imaging of plasmid DNA and viral RNA.
Biomaterials 28, 2403-2411 (2007)
Plasmid DNA and viral RNA were imaged in a liquid environment by dynamic force microscopy (DFM) and fine structures of DNA with heights of 1.82+/-0.66 nm were obtained in topographical images. In simultaneously acquired phase images, DNA could be imaged with better contrast at lower imaging forces. By splitting the cantilever oscillation signal into lower and upper parts, the contribution of the adhesion between tip and sample to the topographical images was eliminated, resulting in better signal-to-noise ratio. DFM of the single stranded RNA genome of a human rhinovirus showed loops protruding from a condensed RNA core, 20-50 nm in height. The mechanical rigidity of the RNA was determined by single molecule pulling experiments. From fitting RNA stretching curves to the Worm-Like-Chain (WLC) model a persistence length of 1.0+/-0.17 nm was obtained.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Atomic force microscope; Dynamic force microscopy; DNA; RNA; Imaging
ISSN (print) / ISBN
0142-9612
e-ISSN
1878-5905
Zeitschrift
Biomaterials
Quellenangaben
Band: 28,
Heft: 15,
Seiten: 2403-2411
Verlag
Elsevier
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Radiation Protection (ISS)