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Zhu, J.Y.* ; Strehle, M.L. ; Frohn, A.* ; Kremmer, E. ; Höfig, K.P. ; Meister, G.* ; Adler, H.

Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68.

J. Virol. 84, 10266-10275 (2010)
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Murine gammaherpesvirus 68 (MHV-68) is closely related to Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) and provides a small-animal model with which to study the pathogenesis of gammaherpesvirus (gammaHV) infections. To completely explore the potential of the MHV-68 system for the investigation of gammaHV microRNAs (miRNAs), it would be desirable to know the number and expression patterns of all miRNAs encoded by MHV-68. By deep sequencing of small RNAs, we systematically investigated the expression profiles of MHV-68 miRNAs in both lytically and persistently infected cells. In addition to the nine known MHV-68 miRNAs, we identified six novel MHV-68 miRNA genes and analyzed the expression levels of all MHV-68 miRNAs. Furthermore, we also characterized the cellular miRNA expression signatures in MHV-68-infected versus noninfected NIH 3T3 fibroblasts and in 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-treated versus nontreated S11 cells. We found that mmu-mir-15b and mmu-mir-16 are highly upregulated upon MHV-68 infection of NIH 3T3 cells, indicating a potential role for cellular miRNAs during MHV-68 infection. Our data will aid in the full exploration of the functions of gammaHV miRNAs.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Animals; Base Sequence; Cell Line; DNA Primers/genetics; Gene Expression Profiling; Genome; Viral; Host-Pathogen Interactions/genetics; Mice; MicroRNAs/chemistry; MicroRNAs/genetics*; NIH 3T3 Cells; Nucleic Acid Conformation; RNA; Viral/chemistry; RNA; Viral/genetics*; Rhadinovirus/genetics*; Rhadinovirus/pathogenicity; Rhadinovirus/physiology; Sequence Analysis; RNA
ISSN (print) / ISBN 0022-538X
e-ISSN 1098-5514
Zeitschrift Journal of Virology
Quellenangaben Band: 84, Heft: 19, Seiten: 10266-10275 Artikelnummer: , Supplement: ,
Verlag American Society for Microbiology (ASM)
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Immunology (IMI)