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Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Expression and localization of axin 2 in colorectal carcinoma and its clinical implication.
Int. J. Colorectal Dis. 28, 1469-1478 (2013)
PURPOSE: Aberrant activation of the Wnt/β-catenin pathway plays a major role in the development of colorectal carcinoma (CRC). Axin 2 is a key protein of this pathway and is upregulated in CRC. Here, we investigated RNA- and protein expression of axin 2 in CRC tissues at the single cell level. Moreover, the association of axin 2 with prognosis and survival was investigated in a large cohort of CRC patients (n = 280). METHODS: Localization and expression of axin 2 and β-catenin was investigated using in situ hybridization and immunohistochemical staining. The quantitative expression levels of axin 2 were determined using RT-qPCR. The association of axin 2 expression with prognosis and survival of the patients was determined by statistical analysis (logrank test, Kaplan-Meier). RESULTS: Our results confirmed the upregulation of axin 2 in CRC and showed that it is broadly expressed in the cytoplasm of the tumor epithelial cells both, in the tumor center and at the invasion front. Axin 2 was rarely expressed by tumor stromal cells and only weakly by normal colonic epithelial cells. Staining of β-catenin and axin 2 in consecutive CRC tissue sections revealed that nuclear translocation of β-catenin in the tumor front was not associated with changes in the cytoplasmic localization of axin 2. Axin 2 did not show any association with proven prognostic factors or survival of the CRC patients. CONCLUSION: The generally increased expression of axin 2 in all tumor stages as compared to normal tissue suggests an initiating pathogenic function in the development of CRC.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Wnt/β-catenin signaling; Axin 2; β-Catenin; Colorectal carcinoma; Wnt Signaling Pathway ; Paraffin-embedded Tissue ; Beta-catenin ; Colon-cancer ; Functional Interaction ; Negative Regulator ; Endothelial-cells ; Breast-cancer ; Stem-cells ; In-vivo
ISSN (print) / ISBN
0179-1958
e-ISSN
1432-1262
Zeitschrift
International Journal of Colorectal Disease
Quellenangaben
Band: 28,
Heft: 11,
Seiten: 1469-1478
Verlag
Springer
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute for Metabolism and Immunology (IMI)