OpenSSL SSL_connect: Connection reset by peer in connection to v2.sherpa.ac.uk:443 PuSH - Publikationsserver des Helmholtz Zentrums München: <em>In vitro</em> monitoring of base excision repair in <em>Saccharomyces cerevisiae</em>.

PuSH - Publikationsserver des Helmholtz Zentrums München

Mörtl, S. ; Ahne, F.

In vitro monitoring of base excision repair in Saccharomyces cerevisiae.

Methods Mol. Biol. 920, 279-287 (2012)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Base excision repair (BER) is an important mechanism to maintain genomic stability. Here we offer a set of protocols to quantitatively analyze BER capacity in whole cell-free yeast extracts. Cell-free yeast extracts were obtained by a French press procedure and repair capacities were measured by using oligonucleotide substrates. Repair products were separated by polyacrylamide gel electrophoresis and detected by autoradiography. These set of methods allow the analysis of different kinds of base damage and of individual mechanistic steps within BER. We used these protocols to investigate a new role of the DNA double strand break repair protein XRS1 in BER (1).
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Herausgeber Bjergbaek, L.*
Korrespondenzautor
Schlagwörter Base excision repair assay; Incision assay; Gap filling assay; Whole cell-free extracts; Radioactive labeling; Oligonucleotide-based assays; Denaturing polyacrylamide gel electrophoresis
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Konferenztitel DNA Repair Protocols
Zeitschrift Methods in Molecular Biology
Quellenangaben Band: 920, Heft: , Seiten: 279-287 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Berlin [u.a.]
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Radiation Biology (ISB)