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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
In vitro monitoring of base excision repair in Saccharomyces cerevisiae.
Methods Mol. Biol. 920, 279-287 (2012)
Base excision repair (BER) is an important mechanism to maintain genomic stability. Here we offer a set of protocols to quantitatively analyze BER capacity in whole cell-free yeast extracts. Cell-free yeast extracts were obtained by a French press procedure and repair capacities were measured by using oligonucleotide substrates. Repair products were separated by polyacrylamide gel electrophoresis and detected by autoradiography. These set of methods allow the analysis of different kinds of base damage and of individual mechanistic steps within BER. We used these protocols to investigate a new role of the DNA double strand break repair protein XRS1 in BER (1).
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Herausgeber
Bjergbaek, L.*
Schlagwörter
Base excision repair assay; Incision assay; Gap filling assay; Whole cell-free extracts; Radioactive labeling; Oligonucleotide-based assays; Denaturing polyacrylamide gel electrophoresis
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Konferenztitel
DNA Repair Protocols
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 920,
Seiten: 279-287
Verlag
Springer
Verlagsort
Berlin [u.a.]
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Radiation Biology (ISB)