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DMSO enhances electrospray response, boosting sensitivity of proteomic experiments.
Nat. Methods 10, 989-991 (2013)
We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Mass-spectrometry ; Peptide Identification ; Nanoelectrospray ; Ionization ; Molecules
ISSN (print) / ISBN
1548-7091
e-ISSN
1548-7105
Zeitschrift
Nature Methods
Quellenangaben
Band: 10,
Heft: 10,
Seiten: 989-991
Verlag
Nature Publishing Group
Verlagsort
New York, NY
Begutachtungsstatus
Peer reviewed