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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Profile identification of disease-associated humoral antigens using AMIDA, a novel proteomics-based technology.
Cell. Mol. Life Sci. 61, 1198-1207 (2004)
We describe AMIDA (autoantibody-mediated identification of antigens), a novel target identification technology based on the immunoprecipitation of disease-specific antigens by autologous serum antibodies followed by two-dimensional electrophoretic separation, and their identification via mass spectrometry. Twenty-seven potential carcinoma antigens were identified including proteins of hitherto unknown function. Validation of one of the identified antigens, cytokeratin 8, revealed its de novo expression in hyperplastic tissue, gradual overexpression with increasing malignancy, and ectopic localization on the cell surface. Furthermore, a strong prevalence of CK8-specific antibodies occurred in the serum of cancer patients already at early disease stages. In situ hybridization for one marker of unknown function, KIAA1273/TOB3, demonstrated its strong overexpression in head and neck carcinomas, thus making it a likely tumor antigen candidate. Eventually, AMIDA could foster significant improvements for the diagnosis and therapy of human diseases eliciting a humoral immune response, and allows for the rapid identification of new target molecules.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Amida ; Ck8 ; Hnscc ; Humoral Antigens ; Kiaa1273/tob3
ISSN (print) / ISBN
1420-682X
e-ISSN
1420-9071
Zeitschrift
Cellular and Molecular Life Sciences - CMLS
Quellenangaben
Band: 61,
Heft: 10,
Seiten: 1198-1207
Verlag
Birkhäuser
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Research Unit Gene Vector (AGV)