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Wollenberg, B.* ; Schmidt, W.* ; Schmitt, B.* ; Lang, S.* ; Zeidler, R.*

Rezeptor-mediierter Gentransfer in Plattenepithelkarzinome des Kopf-Hals-Bereiches.

Receptor mediated gene transfer in squamous epithelial carcinoma of the head-neck area.

Laryngol. Rhinol. Otol. 76, 411-414 (1997)
PMC
BACKGROUND: The "Adeno-Virus-Enhanced-Receptor-Mediated" (AVET)-System uses the receptor-mediated endocytosis route to introduce DNA into mammalian cells. By the help of a replication defective adenovirus with endosomolytic activity the coentry of receptor-bound DNA particles is faciliated. The transport to the nucleus followed by gene expression is multifold enhanced. METHODS: Two different permanent cell lines from head and neck squamous cell cancer were transfected with the reportergene for luciferase with either AVET or different liposoms in vitro. RESULTS: In comparison it becomes obvious, that AVET shows a multifold higher transfection rate in vitro than the liposomal formulations applied. CONCLUSIONS: AVET could be used for efficient transfection of epithelial cells, that are fairly reluctant to DNA transfer. Further trials in the mouse model must prove a possible application in vivo.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 0302-9379
e-ISSN 1438-8685
Zeitschrift Laryngo-Rhino-Otologie
Quellenangaben Band: 76, Heft: 7, Seiten: 411-414 Artikelnummer: , Supplement: ,
Verlag Thieme
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Gene Vector (AGV)