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Mascher, M.* ; Muehlbauer, G.J.* ; Rokhsar, D.S.* ; Chapman, J.* ; Schmutz, J.* ; Barry, K.* ; Muñoz-Amatriaín, M.* ; Close, T.J.* ; Wise, R.P.* ; Schulman, A.H.* ; Himmelbach, A.* ; Mayer, K.F.X. ; Scholz, U.* ; Poland, J.A.* ; Stein, N.* ; Waugh, R.*

Anchoring and ordering NGS contig assemblies by population sequencing (POPSEQ).

Plant J. 76, 718-727 (2013)

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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.

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Next-generation whole-genome shotgun assemblies of complex genomes are highly useful, but fail to link nearby sequence contigs with each other or provide a linear order of contigs along individual chromosomes. Here, we introduce a strategy based on sequencing progeny of a segregating population that allows de novo production of a genetically anchored linear assembly of the gene space of an organism. We demonstrate the power of the approach by reconstructing the chromosomal organization of the gene space of barley, a large, complex and highly repetitive 5.1 Gb genome. We evaluate the robustness of the new assembly by comparison to a recently released physical and genetic framework of the barley genome, and to various genetically ordered sequence-based genotypic datasets. The method is independent of the need for any prior sequence resources, and will enable rapid and cost-efficient establishment of powerful genomic information for many species.

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