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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Two herbivore-induced cytochrome P450 enzymes CYP79D6 and CYP79D7 catalyze the formation of volatile aldoximes involved in poplar defense.
Plant Cell 25, 4737-4754 (2013)
Aldoximes are known as floral and vegetative plant volatiles but also as biosynthetic intermediates for other plant defense compounds. While the cytochrome P450 monooxygenases (CYP) from the CYP79 family forming aldoximes as biosynthetic intermediates have been intensively studied, little is known about the enzymology of volatile aldoxime formation. We characterized two P450 enzymes, CYP79D6v3 and CYP79D7v2, which are involved in herbivore-induced aldoxime formation in western balsam poplar (Populus trichocarpa). Heterologous expression in Saccharomyces cerevisiae revealed that both enzymes produce a mixture of different aldoximes. Knockdown lines of CYP79D6/7 in gray poplar (Populus × canescens) exhibited a decreased emission of aldoximes, nitriles, and alcohols, emphasizing that the CYP79s catalyze the first step in the formation of a complex volatile blend. Aldoxime emission was found to be restricted to herbivore-damaged leaves and is closely correlated with CYP79D6 and CYP79D7 gene expression. The semi-volatile phenylacetaldoxime decreased survival and weight gain of gypsy moth (Lymantria dispar) caterpillars, suggesting that aldoximes may be involved in direct defense. The wide distribution of volatile aldoximes throughout the plant kingdom and the presence of CYP79 genes in all sequenced genomes of angiosperms suggest that volatile formation mediated by CYP79s is a general phenomenon in the plant kingdom.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Caterpillars Malacosoma-disstria; Cyanogenic Glucoside Dhurrin; Manihot-esculenta Crantz; Trichocarpa X Deltoides; Populus-trichocarpa; Lotus-japonicus; Plant Defense; Insect Herbivores; Escherichia-coli; Hybrid Poplar
ISSN (print) / ISBN
1040-4651
e-ISSN
1532-298X
Zeitschrift
The Plant cell
Quellenangaben
Band: 25,
Heft: 11,
Seiten: 4737-4754
Verlag
American Society of Plant Biologists (ASPB)
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed