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Degroote, R.L.* ; Hauck, S.M. ; Treutlein, G.* ; Amann, B.* ; Fröhlich, K.J.H.* ; Kremmer, E. ; Merl, J. ; Stangassinger, M.* ; Ueffing, M. ; Deeg, C. A.*

Expression changes and novel interaction partners of talin 1 in effector cells of autoimmune uveitis.

J. Proteome Res. 12, 5812–5819 (2013)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Autoimmune uveitis is characterized by crossing of blood-retinal barrier (BRB) by autoaggressive immune cells. Equine recurrent uveitis (ERU) is a valuable spontaneous model for autoimmune uveitis and analyses of differentially expressed proteins in ERU unraveled changed protein clusters in target tissues and immune system. Healthy eyes are devoid of leukocytes. In ERU, however, leukocytes enter the inner eye and subsequently destroy it. Molecular mechanisms enabling cell migration through BRB still remain elusive. Previously, we detected decreased talin 1 expression in blood-derived granulocytes of ERU cases, linking the innate immune system to ERU. Because changes in leukocyte protein expression pattern may play a role in pathological abnormalities leading to migration ability, we aimed at identifying interactors of talin 1 in leukocytes with immunoprecipitation, followed by LC-MS/MS for candidate identification. This enabled us to identify CD90 (Thy1) as novel interactor of talin 1 besides several other interactors. In blood-derived granulocytes from healthy individuals, CD90 was highly abundant and significantly reduced in ERU, especially in effector cells. Connection between talin 1 and CD90 and their expression differences in inflammation is an interesting novel finding allowing deeper insight into immune response of innate immune system and granulocyte migration ability in this organ-specific autoimmune disease.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Protein interaction; cell migration; Autoimmune; Spontaneous uveitis; Granolytes; Talin 1; CD90; Equine Recurrent Uveitis ; Integrin Activation ; Label-free ; Thy-1 ; Protein ; Horses ; Migration ; Immunity ; Binding ; Innate
Sprache englisch
Veröffentlichungsjahr 2013
HGF-Berichtsjahr 2013
ISSN (print) / ISBN 1535-3893
e-ISSN 1535-3907
Zeitschrift Journal of Proteome Research
Quellenangaben Band: 12, Heft: 12, Seiten: 5812–5819 Artikelnummer: , Supplement: ,
Verlag American Chemical Society (ACS)
Begutachtungsstatus Peer reviewed
Institut(e) CF Metabolomics & Proteomics (CF-MPC)
Institute of Molecular Immunology (IMI)
POF Topic(s) 30203 - Molecular Targets and Therapies
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Forschungsfeld(er) Enabling and Novel Technologies
Immune Response and Infection
PSP-Element(e) G-505700-001
G-501793-001
PubMed ID 24144192
DOI 10.1021/pr400837f
WOS ID WOS:000328231300040
Scopus ID 84890065334
Erfassungsdatum 2013-11-15
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