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Fernsebner, K. ; Zorn, J. ; Kanawati, B. ; Walker, A. ; Michalke, B.

Manganese leads to an increase in markers of oxidative stress as well as to a shift in the ratio of Fe(ιι)/(ιιι) in rat brain tissue.

Metallomics 6, 921-931 (2014)
Verlagsversion Volltext DOI PMC
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Occupationally or environmentally caused chronic exposure to Manganese (Mn) can lead to a degeneration of dopaminergic neurons inducing a Parkinson-like complaint called manganism. Deciphering the ongoing neurodegenerative mechanisms in the affected brain is still a major task for understanding the complex modes of action. Therefore, we applied a non-toxic, oral feeding in rats simulating a chronic exposure to Mn. Analysis of brain extracts by electrospray ionization Fourier transform resonance mass spectrometry (ESI-FT-ICR-MS) revealed an increase in markers of oxidative stress like glutathione disulfide (GSSG), prostaglandins, and 15(S)-HETE, a marker of lipid peroxidation. Furthermore, acetylcholinesterase (AchE) activity and glutamate concentrations were elevated in brain samples of Mn-supplemented rats, suggesting oxidative stress in the brain tissue. Application of ion chromatography coupled to inductively coupled plasma-optical emission spectrometry (IC-ICP-OES) further showed a shift of Fe(iii) towards Fe(ii) in the brain samples enabling for example the action of the Fenton reaction. This is the first time that changes in the Fe-species distribution could be related to Mn-induced neuroinflammation and is therefore enlarging the knowledge of this complex neurodegenerative condition. The combination of our findings provides substantial evidence that Mn-induced neuroinflammation leads to oxidative stress triggered by multifactorial pathophysiological processes.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Central-nervous-system; Alzheimers-disease; Induced Neurotoxicity; Nitric-oxide; In-vitro; Choline-acetyltransferase; Parkinsons-disease; Glutamate; Iron; Exposure
ISSN (print) / ISBN 1756-5901
e-ISSN 1756-591X
Zeitschrift Metallomics
Quellenangaben Band: 6, Heft: 4, Seiten: 921-931 Artikelnummer: , Supplement: ,
Verlag Royal Society of Chemistry (RSC)
Verlagsort Cambridge
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit BioGeoChemistry and Analytics (BGC)
CF Comparative Medicine (AVM)