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Frommberger, M. ; Hertkorn, N. ; Englmann, M. ; Jakoby, S. ; Hartmann, A. ; Kettrup, A. ; Schmitt-Kopplin, P.

Analysis of N-acylhomoserine lactones after alkaline hydrolysis and anion-exchange solid-phase extraction by capillary zone electrophoresis-mass spectrometry.

Electrophoresis 26, 1523-1532 (2005)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A quantitative, specific, and sensitive method for the determination of N-acylhomoserine lactones (HSLs – a group of bacterial semiochemicals) in the form of their hydrolysis products (N-acylhomoserines, HSs) is presented. Real samples were analyzed by capillary zone electrophoresis-mass spectrometry (CZE-MS) after alkaline lactonolysis and extraction by mixed-mode anion-exchange solid-phase extraction. The presented cleanup significantly speeds up the HSL extraction procedure, strongly reduces sample consumption, and is more selective compared to the commonly used liquid/liquid extraction. Completeness of the hydrolysis reaction was examined by nuclear magnetic resonance spectroscopy. This CZE-MS method complements recently published capillary separation techniques (nano liquid chromatography-MS, partial-filling micellar electrokinetic chromatography-MS, gas chromatography-MS) and provides a possibility to differentiate quantitatively between the homoserines (as naturally occurring degradation products) besides the intact homoserine lactones. The method was found to be quantitative down to a concentration of 0.05 µg/mL (limit of quantification), while the limit of detection was determined with 0.01 µg/mL – sufficient for the analysis of culture supernatants.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter capillary electrophoresis; homoserine lactones; mass spectrometry; quorum sensing; solid-phase extraction
ISSN (print) / ISBN 0173-0835
e-ISSN 1522-2683
Zeitschrift Electrophoresis
Quellenangaben Band: 26, Heft: 7-8, Seiten: 1523-1532 Artikelnummer: , Supplement: ,
Verlag Wiley
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Ecological Chemistry (IOEC)
Institute of Soil Ecology (IBOE)