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Qiu, S. ; Gözdereliler, E. ; Weyrauch, P. ; Lopez, E.C. ; Kohler, H.P.* ; Sørensen, S.R.* ; Meckenstock, R.U. ; Elsner, M.

Small 13C/12C fractionation contrasts with large enantiomer fractionation in aerobic biodegradation of phenoxy acids.

Environ. Sci. Technol. 48, 5501-5511 (2014)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Phenoxy acid herbicides are important groundwater contaminants. Stable isotope analysis and enantiomer analysis are well-recognized approaches for assessing in situ biodegradation in the field. In an aerobic degradation survey with six phenoxyacetic acid and three phenoxypropionic acid-degrading bacteria we measured (a) enantiomer-specific carbon isotope fractionation of MCPP ((R,S)-2-(4-chloro-2-methylphenoxy)-propionic acid), DCPP ((R,S)-2-(2,4-dichlorophenoxy)-propionic acid), and 4-CPP ((R,S)-2-(4-chlorophenoxy)-propionic acid); (b) compound-specific isotope fractionation of MCPA (4-chloro-2-methylphenoxyacetic acid) and 2,4-D (2,4-dichlorophenoxyacetic acid); and (c) enantiomer fractionation of MCPP, DCPP, and 4-CPP. Insignificant or very slight (ε = -1.3‰ to -2.0‰) carbon isotope fractionation was observed. Equally small values in an RdpA enzyme assay (εea = -1.0 ± 0.1‰) and even smaller fractionation in whole cell experiments of the host organism Sphingobium herbicidovorans MH (εwc = -0.3 ± 0.1‰) suggest that (i) enzyme-associated isotope effects were already small, yet (ii) further masked by active transport through the cell membrane. In contrast, enantiomer fractionation in MCPP, DCPP, and 4-CPP was pronounced, with enantioselectivities (ES) of -0.65 to -0.98 with Sphingomonas sp. PM2, -0.63 to -0.89 with Sphingobium herbicidovorans MH, and 0.74 to 0.97 with Delftia acidovorans MC1. To detect aerobic biodegradation of phenoxypropionic acids in the field, enantiomer fractionation seems, therefore, a stronger indicator than carbon isotope fractionation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Sphingomonas-herbicidovorans Mh; Stable-isotope Fractionation; 2,4-dichlorophenoxyacetic Acid; Organic Contaminants; Natural Attenuation; Degrading Bacteria; Ground-water; Enantioselective Degradation; Limestone Aquifer; Chiral Herbicide
ISSN (print) / ISBN 0013-936X
e-ISSN 1520-5851
Zeitschrift Environmental Science & Technology
Quellenangaben Band: 48, Heft: 10, Seiten: 5501-5511 Artikelnummer: , Supplement: ,
Verlag American Chemical Society (ACS)
Verlagsort Washington, DC
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Groundwater Ecology (IGOE)