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Schlicke, H.* ; Hartwig, A.S.* ; Firtzlaff, V.* ; Richter, A.S.* ; Glässer, C.* ; Mayer, K.F.X. ; Finkemeier, I.* ; Grimm, B.*

Induced deactivation of genes encoding chlorophyll biosynthesis enzymes disentangles tetrapyrrole-mediated retrograde signalling.

Mol. Plant 7, 1211-1227 (2014)
Verlagsversion Volltext DOI PMC
Closed
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
In photosynthetic organisms, tetrapyrrole-mediated retrograde signals are proposed to contribute to a balanced nuclear gene expression (NGE) in response to metabolic activity in chloroplasts. We followed an experimental short-term approach that allowed the assessment of modified NGE during the first hours of specifically modified enzymatic steps of the Mg branch of tetrapyrrole biosynthesis, when pleiotropic effects of other signals can be avoided. In response to 24 h-induced silencing of CHLH, CHLM and CHL27 encoding the CHLH subunit of Mg chelatase, the Mg protoporphyrin methyltransferase and Mg protoporphyrin monomethylester cyclase, respectively, deactivated gene expression rapidly led to reduced activity of the corresponding enzymes and altered Mg porphyrin levels. But NGE was not substantially altered. When these three genes were continuously inactivated for up to 4 days, changes of transcript levels of nuclear genes were determined. CHL27 silencing for more than 24 h results in necrotic leaf lesions and modulated transcript levels of oxidative stress-responsive and photosynthesis-associated nuclear genes (PhANGs). The prolonged deactivation of CHLH and CHLM results in slightly elevated transcript levels of PhANGs and tetrapyrrole-associated genes. These time-resolved studies indicate a complex scenario for the contribution of tetrapyrrole biosynthesis on NGE mediated by (1)O2-induced signalling and feedback-regulated ALA synthesis.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Mg Protoporphyrin ; Plastid Signal ; Retrograde Signal ; Tetrapyrrole Metabolism ; Transcription Analysis; Mg-protoporphyrin-ix; Singlet Oxygen; Arabidopsis-thaliana; Chloroplast Development; Aminolevulinic-acid; Oxidative Stress; Plastid Signals; Redox Signals; De-etiolation; Nuclear Genes
ISSN (print) / ISBN 1674-2052
e-ISSN 1752-9867
Zeitschrift Molecular Plant
Quellenangaben Band: 7, Heft: 7, Seiten: 1211-1227 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Oxford
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Plant Genome and Systems Biology (PGSB)