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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
CHD4/Mi-2 beta activity is required for the positioning of the mesoderm/neuroectoderm boundary in Xenopus
Genes Dev. 21, 973-983 (2007)
Experiments in Xenopus have illustrated the importance of extracellular morphogens for embryonic gene regulation in vertebrates. Much less is known about how induction leads to the correct positioning of boundaries; for example, between germ layers. Here we report that the neuroectoderm/mesoderm boundary is controlled by the chromatin remodeling ATPase CHD4/Mi-2beta. Gain and loss of CHD4 function experiments shifted this boundary along the animal-vegetal axis at gastrulation, leading to excess mesoderm formation at the expense of neuroectoderm, or vice versa. This phenotype results from specific alterations in gene transcription, notably of the neural-promoting gene Sip1 and the mesodermal regulatory gene Xbra. We show that CHD4 suppresses Sip1 transcription by direct binding to the 5' end of the Sip1 gene body. Furthermore, we demonstrate that CHD4 and Sip1 expression levels determine the "ON" threshold for Nodal-dependent but not for eFGF-dependent induction of Xbra transcription. The CHD4/Sip1 epistasis thus constitutes a regulatory module, which balances mesoderm and neuroectoderm formation.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
CHD4/Mi-2beta; Sip1; Xbra; chromatin remodeling; Activin induction threshold; germ layer
ISSN (print) / ISBN
0890-9369
e-ISSN
1549-5477
Zeitschrift
Genes and Development
Quellenangaben
Band: 21,
Heft: 8,
Seiten: 973-983
Verlag
Cold Spring Harbor Laboratory Press
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Molecular Immunology (IMI)