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Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level.
Analyst 139, 5709-5717 (2014)
Studying cell-to-cell heterogeneity requires techniques which robustly deliver reproducible results with single-cell sensitivity. Through a new fabrication method for the microarrays for mass spectrometry (MAMS) platform, we now have attained robustness and reproducibility in our single-cell level mass spectrometry measurements that allowed us to combine single-cell MAMS-based measurements from different days and samples. By combining multiple measurements, we were able to identify three co-existing phenotypes in an isogenic population of Saccharomyces cerevisiae characterized by distinctively different levels of glycolytic intermediates.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Times Cited
Scopus
Cited By
Cited By
Altmetric
3.906
1.119
14
14
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Assisted-laser-desorption/ionization; Mass-spectrometry; Yeast-cells; Metabolomics; Heterogeneity; Matrix; Metabolism; Switch; Flux
Sprache
englisch
Veröffentlichungsjahr
2014
HGF-Berichtsjahr
2014
ISSN (print) / ISBN
0003-2654
e-ISSN
1364-5528
Zeitschrift
Analyst, The
Quellenangaben
Band: 139,
Heft: 22,
Seiten: 5709-5717
Verlag
Royal Society of Chemistry (RSC)
Verlagsort
Cambridge
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Computational Biology (ICB)
POF Topic(s)
30205 - Bioengineering and Digital Health
Forschungsfeld(er)
Enabling and Novel Technologies
PSP-Element(e)
G-503800-001
PubMed ID
25221792
WOS ID
WOS:000344310500012
Scopus ID
84907978322
Erfassungsdatum
2014-10-09