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Jha, B.* ; Thakur, M.C.* ; Gontia, I.* ; Albrecht, V. ; Stoffels, M. ; Schmid, M. ; Hartmann, A.

Isolation, partial identification and application of diazotrophic rhizobacteria from traditional Indian rice cultivars.

Eur. J. Soil Biol. 45, 62-72 (2009)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A diversity of N-2-fixing (diazotrophic) bacteria was isolated from two traditional rice cultivars, Sataria and Kartiki, from the rice growing area of Mithila region of North Bihar, India, where low levels of nitrogen fertilizers are applied. Nitrogen-free semisolid media NFb, JMV and LGI with different carbon sources and pH-values were used for enrichment and isolation of root-associated diazotrophs. The colonization density of roots by diazotrophs, as estimated from positive pellicle formation at highest dilution in nitrogen-free enrichment media, was 10(6)-10(8) diazotrophic bacteria per g fresh root weight. Roots of the cultivar Kartiki were found to be more densely colonized endophytically by diazotrophs as detected after chloramine T (1%) surface disinfection. To ascertain the phylogenetic affiliation of the isolates, phylogenetic oligonucleotide probes and the Fluorescent in situ Hybridization (FISH) technique were applied. Using group-specific rRNA directed oligonucleotide probes, the majority of the isolates could be identified as alpha-, beta-, or gamma-proteobacteria. Using 16S and 23S rRNA-directed genus- or species-specific probes, Herbaspirillum seropedicae, Azospirillum amazonense, Burkholderia cepacia/vietnamiensis, Rhizobia and Pseudomonas spp. were found to be the most prominent root associated culturable diazotrophs. Diazotrophic Gluconacetobacter spp. were also demonstrated as colonizers of rice roots. Burkholderia cenocepacia, Pseudomonas sp. and three diazotrophic PGPR reference strains were used for the inoculation of axenically grown rice seedlings to determine the plant growth promoting potential. Significant increases in the shoot length (up to 60%), shoot dry weight (up to 33%) and the grain yield (up to 26%) per plant were observed in non-axenic pot and field trials. Using semisolid enrichment media after surface sterilization of field grown inoculated rice roots and oligonucleotide probing of the diazotrophic enrichment cultures, a sustainable colonization with the inoculated bacteria could be demonstrated.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Diazotrophic bacteria; Endophytic colonization; Phylogenetic oligonucleotide probes; Plant growth and yield promotion; Rice; leguminosarum bv trifolii; gram-positive bacteria; in-situ detection; dna g+c content; sugar-cane; oligonucleotide probes; wetland rice; phylogenetic trees; root colonization; nitrogen-fixation
ISSN (print) / ISBN 1164-5563
e-ISSN 1778-3615
Zeitschrift European Journal of Soil Biology
Quellenangaben Band: 45, Heft: 1, Seiten: 62-72 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Paris
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Microbe-Plant Interactions (AMP)