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Generation of targeted mouse mutants by embryo microinjection of TALENs.
Methods 69, 94-101 (2014)
Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step, without the need for embryonic stem cells. Thereby, knockout and knockin alleles can be generated fast and efficiently by embryo microinjection of TALEN mRNAs and targeting vectors. In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Knockin ; Knockout ; One-cell Embryo ; Gene Targeting ; Talen ; Mouse; Zinc-finger Nucleases; Double-strand Breaks; Homologous Recombination; Dna Cleavage; Effector Nucleases; Mammalian-cells; Gene Disruption; Iii Effectors; Genome; Mice
ISSN (print) / ISBN
1046-2023
e-ISSN
1095-9130
Zeitschrift
Methods
Quellenangaben
Band: 69,
Heft: 1,
Seiten: 94-101
Verlag
Elsevier
Verlagsort
Amsterdam [u.a.]
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Developmental Genetics (IDG)