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Mao, Z.* ; Quintanilla-Martinez, L. ; Raffeld, M.* ; Richter, M. ; Krugmann, J.* ; Burek, C.* ; Hartmann, E.* ; Rudiger, T.* ; Jaffe, E.S.* ; Müller-Hermelink, H.K.* ; Ott, G.* ; Fend, F.* ; Rosenwald, A.*

IgVH mutational status and clonality analysis of Richter's transformation: Diffuse large B-cell lymphoma and Hodgkin lymphoma in association with B-cell chronic lymphocytic leukemia (B-CLL) represent 2 different pathways of disease evolution.

Am. J. Surg. Pathol. 31, 1605-1614 (2007)
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Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Approximately 5% of B-cell chronic lymphocytic leukemia (B-CLL) patients develop a secondary aggressive lymphoma, usually of diffuse large B-cell type (DLBCL), termed Richter's transformation (RT). Rarely, classic Hodgkin lymphoma (HL) is observed. Published small series suggest that tumor cells in DLBCL and HL can be clonally identical to the B-CLL clone or arise as an independent, secondary lymphoma. We describe the morphology, immunophenotype, and clinical features of 34 classic RT patients with DLBCL, 6 cases of B-CLL with HL, and 8 cases with scattered CD30-positive Hodgkin and Reed-Sternberg (HRS)-like cells. The clonal relationship of the 2 components was analyzed using sequencing analysis of immunoglobulin heavy chain variable region (IgVH) genes. In classic RT, 18/23 B-CLL cases (78%) showed clonal progression to DLBCL with identical IgVH sequences in both lymphoma components, whereas in 5 cases (22%) the DLBCL was clonally unrelated. Among clonally related RT samples, 73% carried unmutated IgVH genes, whereas 4/5 unrelated cases were mutated. Immunophenotypically, most cases of DLBCL irrespective of clonal relatedness showed significant differences in phenotype compared with the B-CLL, with common loss of CD5 and CD23. Using immuno-laser capture microdissection, sequencing of the IgVH CDR3 region of isolated HRS cells showed that 2/2 cases with HL were clonally unrelated, whereas they were clonally identical in 1/2 cases of B-CLL with scattered HRS-like cells. HRS or HRS-like cells in all 3 unrelated cases showed evidence of Epstein-Barr virus infection. Of interest, 5/6 cases of B-CLL with HL, and 5/6 cases of B-CLL with HRS cells showed mutated IgVH genes.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Richter's transformation; clonality analysis; IgH mutational analysis; B-cell chronic lymphocyte leukemia; Hodgkin lymphoma; microdissection
Sprache englisch
Veröffentlichungsjahr 2007
HGF-Berichtsjahr 2007
ISSN (print) / ISBN 0147-5185
e-ISSN 1532-0979
Zeitschrift American Journal of Surgical Pathology, The
Quellenangaben Band: 31, Heft: 10, Seiten: 1605-1614 Artikelnummer: , Supplement: ,
Verlag Lippincott Williams & Wilkins
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pathology (PATH)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-500300-001
DOI 10.1097/PAS.0b013e31804bdaf8
Scopus ID 34748861564
Erfassungsdatum 2007-10-22
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