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Schulze, S.* ; Bahnweg, G.

Nachweis der Gattung Armillaria (Fr.: Fr.) Staude und Heterobasidion annosum (Fr.) Bref. in Fichte (Picea abies [L.] Karst.) und Erfassung der klonalen ausbreitung von A. ostoyae-Genotypen unter Verwendung molekularer Methoden.

Identification of the genus Armillaria (Fr.: Fr.) Staude and Heterobasidion annosum (Fr.) Bref. in Norway spruce (Picea abies [L.] Karst.) and determination of clonal distribution of A. ostoyae-genolypes by molecular methods.

Eur. J. For. Res. 117, 98-114 (1998)
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Methods based on the polymerase chain reaction (PCR) for generating molecular markers are illustrated by two important forest pathogens, Armillaria spp. and H. annosum. Using the primer pairs ARM-1/ARM-2 and HET-7/HET-8, derived from sequences of the "internal transcribed spacer" (ITS) regions of the rDNA repeat, Armillaria spp.- and H. annosum-specific DNA fragments were amplified by PCR. This PCR-based detection method allows a rapid and definite diagnosis of both important root and butt rot pathogens in different substrates or plant tissues, especially in early stages. Genetic variability among 20 A. ostoyae-isolates from different geographical origins was studied. UPGMA cluster analysis of random amplified polymorphic DNAs (RAPDs) profiles generated by 10 decamer random primers (OPA 01-10) grouped the isolates in subclusters at similarity levels between 40% and 96%, indicating high intraspecific genetic variation. The potential role of historical and current spread of spruce plants on the genetic variation of A. ostoyae in Europe is discussed. The established polymorphic DNA markers were used to determine the population structure, dynamics and spatial distribution of A. ostoyae-"genets" in areas colonized by the fungus. First investigations revealed different distribution strategies of A. ostoyae, which may be mediated by climatic factors, location (e.g., soil characters), and pollutants.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Armillaria Spp. ; Heterobasidion Annosum; Diagnosis; Its-pcr-primer ; Population Structure ; Rapds
ISSN (print) / ISBN 1612-4669
e-ISSN 1612-4677
Zeitschrift European Journal of Forest Research
Quellenangaben Band: 117, Heft: 2, Seiten: 98-114 Artikelnummer: , Supplement: ,
Verlag Springer
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biochemical Plant Pathology (BIOP)