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Pfitzner, J. ; Schlegel, H.G.

Denitrifikation bei Hydrogenomonas eutropha Stamm H16.

Denitrification in Hydrogenomonas eutropha strain H16.

Arch. Microbiol. 90, 199-211 (1973)
Verlagsversion DOI
The hydrogen bacterium Hydrogenomonas eutropha (syn. Alcaligenes eutrophus) strain H 16 is able to grow anaerobically with fructose and nitrate or nitrite, respectively. Autotrophic anaerobic growth under a gas atmosphere of hydrogen and carbon dioxide (90+10 vol-%) with nitrate as the sole hydrogen acceptor is minimal. During anaerobic growth with nitrate as H-acceptor, two growth phases are distinguishable: During the first phase cell growth occurs with the reduction of nitrate to nitrite, which is accumulated; on the second phase nitrite is reduced with the formation of gaseous nitrogen. Washed, anaerobically grown cells reduce nitrate and nitrite with the formation of N2. Stoichiometric experiments employing hydrogen or fructose as the hydrogen donors are consistent with the conclusion that nitrogen is the sole product of denitrification by these cells. This was confirmed by mass spectrometric analysis of the gas formed. Aerobically grown cells are able to reduce nitrate only to nitrite; when grown in the presence of ammonia, the reduction rate is very low. The results indicate that strain H 16 contains only one nitrate reductase. The formation of this enzyme system is not influenced by oxygen, however, is repressed by ammonia. When employing a purified soluble fraction and particles, nitrite reductases were found in both fractions. The nitrite reductase system is formed only under anaerobic conditions.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 0003-9276
e-ISSN 1432-072X
Zeitschrift Archives of Microbiology
Quellenangaben Band: 90, Heft: 3, Seiten: 199-211 Artikelnummer: , Supplement: ,
Verlag Springer
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institut für Mikrobiologie