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Eyerich, K. ; Böckelmann, R.* ; Pommer, A.J.* ; Foerster, S. ; Hofmeister, H.* ; Huss-Marp, J. ; Cavani, A.* ; Behrendt, H. ; Ring, J. ; Gollnick, H.* ; Bonnekoh, B.* ; Traidl-Hoffmann, C.

Comparative in situ topoproteome analysis reveals differences in patch test-induced eczema: Cytotoxicity-dominated nickel versus pleiotrope pollen reaction.

Exp. Dermatol. 19, 511-517 (2010)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A subgroup of patients with atopic eczema develops acute eczematous reactions to type I allergy-inducing agents such as pollen that clinically resemble type IV allergies induced by haptens like metal ions. To clarify the underlying immunologic mechanisms, this study was designed to map the inflammatory in situ topoproteome of eczematous responses to grass/birch pollen and nickel by using atopy patch test (APT) and nickel patch test (NPT) as an appropriate clinical model, respectively. Biopsies from NPT (n = 6) and APT (n = 6) with positive reactions at 72 h were analysed by multiple epitope ligand cartography (MELC), which enabled to investigate coexpression of 49 different epitopes immunohistochemically in a single given tissue section. Colocalisation of IgE and FcepsilonRI was investigated by confocal microscopy. Compared with APT responses, NPT reactions were dominated by cytotoxic TIA-1 + and CD8 + T cells. In contrast, the immune response in APT reactions appeared more pleiotrope - as detected by colocalisation analysis. Multiple combinatorial molecular phenotype (CMP) motifs containing naive, early maturation and memory T cell (CD45RA, CD7, CD44, CD45R0), and general activation markers (CLA, HLA-DR, CD13, CD29, CD58, CD71, CD138) were significantly higher expressed in APT when compared with NPT reactions. APT response was confirmed to be accompanied by IgE bound to FcepsilonRI. In summary, our results demonstrate that the NPT reaction is clearly dominated by cytotoxic events, while the APT reaction to pollen grains is more heterogeneous and elicits a combined humoral and cellular immune reaction.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter atopic dermatitis/eczema; atopy patch test; in situ topoproteome analysis; multiple epitope ligand cartography; nickel; pollen
ISSN (print) / ISBN 0906-6705
e-ISSN 1600-0625
Zeitschrift Experimental Dermatology
Quellenangaben Band: 19, Heft: 6, Seiten: 511-517 Artikelnummer: , Supplement: ,
Verlag Wiley
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Lung Health and Immunity (LHI)
Institute of Epidemiology (EPI)