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Brühl, P.C. ; Mergenthaler, H.G. ; Ellwart, J.W. ; Dörmér, P.G.

HLA-class II antigens on hemopoietic and stromal cells in human micro long-term bone marrow cultures.

Exp. Hematol. 18, 103-108 (1990)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Using a complement-dependent cytotoxicity assay (CDC), we analyzed the presence of HLA-class II antigens on both stromal and hemopoietic cells in a miniaturized human long-term bone marrow culture system. 4-Hydroperoxycyclophosphamide (4-HC)-resistant hemopoietic stem cells capable of restoring in vitro hemopoiesis on irradiated stromal cell layers were HLA-DR, -DP, and -DQ negative. In addition, these cells failed to bind the monoclonal antibody (mAb) Tu 39, previously proposed as a candidate for the recognition of a novel class II antigen, '-DY'. On the other hand, the formation of confluent stromal cell layers was inhibited by HLA-DR- or -DP-specific mAbs, but not by the HLA-DQ-specific mAb Tu 22. This suggests the presence of HLA-DR- and/or HLA-DP-positive, but HLA-DQ-negative stromal precursor cells.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Hemopoietic Stem Cells ; Hla Antigens ; Long-term Bone Marrow Culture ; Monoclonal Antibodies ; Stromal Cells
ISSN (print) / ISBN 0301-472X
e-ISSN 0301-472X
Zeitschrift Experimental Hematology
Quellenangaben Band: 18, Heft: 2, Seiten: 103-108 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed
Institut(e) Institut für Experimentelle Hämatologie